Contrasting common measures of arbuscular mycorrhizal fungal root colonization.
Identifieur interne : 000603 ( Main/Exploration ); précédent : 000602; suivant : 000604Contrasting common measures of arbuscular mycorrhizal fungal root colonization.
Auteurs : Vasilis Kokkoris [Canada] ; Antreas Pogiatzis [Canada] ; Miranda M. Hart [Canada]Source :
- Journal of microbiological methods [ 1872-8359 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Lin, Racines de plante.
- méthodes : Microscopie, Photographie (méthode), Traitement d'image par ordinateur.
- physiologie : Mycorhizes.
- Symbiose.
English descriptors
- KwdEn :
- MESH :
- methods : Image Processing, Computer-Assisted, Microscopy, Photography.
- microbiology : Flax, Plant Roots.
- physiology : Mycorrhizae.
- Symbiosis.
Abstract
Estimating the abundance of arbuscular mycorrhizal fungi relies entirely on indirect methods, meaning all measures are associated with some variability. The most common methods use microscopic estimates of the relative proportion of root length colonized by fungal structures. These methods typically examine root subsamples. While such methods are inexpensive and relatively simple, significant variation within single root system means there is opportunity for sampling bias. We evaluated the two most common methods of percent root length colonization for AM fungi both as a subsample and for the entire root system of flax plants. We compared these measures to a novel technique that returns projected fungal surface area (fungal coverage), by using microphotography and imaging analysis. Both microscopic methods overestimated the colonization intensity compared to image analysis. Among the microscopic methods, the method which incorporated colonization intensity (Trouvelot) was significantly more similar to imaging method results, than the method that is based on the presence/absence of the fungus (McGonigle).
DOI: 10.1016/j.mimet.2019.105727
PubMed: 31629912
Affiliations:
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Electronic address: bill.kokkoris@ubc.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Pogiatzis, Antreas" sort="Pogiatzis, Antreas" uniqKey="Pogiatzis A" first="Antreas" last="Pogiatzis">Antreas Pogiatzis</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Hart, Miranda M" sort="Hart, Miranda M" uniqKey="Hart M" first="Miranda M" last="Hart">Miranda M. 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Hart</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></analytic><series><title level="j">Journal of microbiological methods</title><idno type="eISSN">1872-8359</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Flax (microbiology)</term><term>Image Processing, Computer-Assisted (methods)</term><term>Microscopy (methods)</term><term>Mycorrhizae (physiology)</term><term>Photography (methods)</term><term>Plant Roots (microbiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Lin (microbiologie)</term><term>Microscopie (méthodes)</term><term>Mycorhizes (physiologie)</term><term>Photographie (méthode) (méthodes)</term><term>Racines de plante (microbiologie)</term><term>Symbiose (MeSH)</term><term>Traitement d'image par ordinateur (méthodes)</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Image Processing, Computer-Assisted</term><term>Microscopy</term><term>Photography</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lin</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Flax</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie</term><term>Photographie (méthode)</term><term>Traitement d'image par ordinateur</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Symbiose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Estimating the abundance of arbuscular mycorrhizal fungi relies entirely on indirect methods, meaning all measures are associated with some variability. The most common methods use microscopic estimates of the relative proportion of root length colonized by fungal structures. These methods typically examine root subsamples. While such methods are inexpensive and relatively simple, significant variation within single root system means there is opportunity for sampling bias. We evaluated the two most common methods of percent root length colonization for AM fungi both as a subsample and for the entire root system of flax plants. We compared these measures to a novel technique that returns projected fungal surface area (fungal coverage), by using microphotography and imaging analysis. Both microscopic methods overestimated the colonization intensity compared to image analysis. Among the microscopic methods, the method which incorporated colonization intensity (Trouvelot) was significantly more similar to imaging method results, than the method that is based on the presence/absence of the fungus (McGonigle).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31629912</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>22</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-8359</ISSN><JournalIssue CitedMedium="Internet"><Volume>167</Volume><PubDate><Year>2019</Year><Month>12</Month></PubDate></JournalIssue><Title>Journal of microbiological methods</Title><ISOAbbreviation>J Microbiol Methods</ISOAbbreviation></Journal><ArticleTitle>Contrasting common measures of arbuscular mycorrhizal fungal root colonization.</ArticleTitle><Pagination><MedlinePgn>105727</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0167-7012(19)30658-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mimet.2019.105727</ELocationID><Abstract><AbstractText>Estimating the abundance of arbuscular mycorrhizal fungi relies entirely on indirect methods, meaning all measures are associated with some variability. The most common methods use microscopic estimates of the relative proportion of root length colonized by fungal structures. These methods typically examine root subsamples. While such methods are inexpensive and relatively simple, significant variation within single root system means there is opportunity for sampling bias. We evaluated the two most common methods of percent root length colonization for AM fungi both as a subsample and for the entire root system of flax plants. We compared these measures to a novel technique that returns projected fungal surface area (fungal coverage), by using microphotography and imaging analysis. Both microscopic methods overestimated the colonization intensity compared to image analysis. Among the microscopic methods, the method which incorporated colonization intensity (Trouvelot) was significantly more similar to imaging method results, than the method that is based on the presence/absence of the fungus (McGonigle).</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kokkoris</LastName><ForeName>Vasilis</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada. Electronic address: bill.kokkoris@ubc.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pogiatzis</LastName><ForeName>Antreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hart</LastName><ForeName>Miranda M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Department of Biology, University of British Columbia, Okanagan campus, 3333 University Way, Kelowna, BC V1V 1V7, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Microbiol Methods</MedlineTA><NlmUniqueID>8306883</NlmUniqueID><ISSNLinking>0167-7012</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019597" MajorTopicYN="N">Flax</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007091" MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="N">Microscopy</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010781" MajorTopicYN="N">Photography</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Arbuscular mycorrhizal fungi</Keyword><Keyword MajorTopicYN="Y">Digital image analysis</Keyword><Keyword MajorTopicYN="Y">Fungal colonization</Keyword><Keyword MajorTopicYN="Y">McGonigle method</Keyword><Keyword MajorTopicYN="Y">Root colonization</Keyword><Keyword MajorTopicYN="Y">Trouvelot method</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31629912</ArticleId><ArticleId IdType="pii">S0167-7012(19)30658-X</ArticleId><ArticleId IdType="doi">10.1016/j.mimet.2019.105727</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Kokkoris, Vasilis" sort="Kokkoris, Vasilis" uniqKey="Kokkoris V" first="Vasilis" last="Kokkoris">Vasilis Kokkoris</name></region><name sortKey="Hart, Miranda M" sort="Hart, Miranda M" uniqKey="Hart M" first="Miranda M" last="Hart">Miranda M. Hart</name><name sortKey="Pogiatzis, Antreas" sort="Pogiatzis, Antreas" uniqKey="Pogiatzis A" first="Antreas" last="Pogiatzis">Antreas Pogiatzis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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